Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. A. Accession codes. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. With a wavelength range from 340 to 850 nm, it is the equivalent to having 510 free filters. 4. This manual is subject to change without notice. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. 0200-6117. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,Get the most out of SoftMax Pro. Molecular Devices VersaMax Microplate Reader Molecular Devices VersaMax Microplate Reader Specifications. A standard curve, provided by the manufacturer, was generated and the sample concentrations determined by 4-parameter logistic curve. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Cell viability was represented by percentage values compared to a. Results are presented as the geometric mean and are derived from at least three experiments. +06-6399-8211 Fax +06-6399-8212 ©2002 Molecular Devices Corporation. For more information, please visit us at With the VersaMax microplate reader, you will never have to buy another filter. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. ¨Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. Antioxidant enzyme activityProtein concentration was determined from diluted crude venom and nanoparticle samples (1 mg/mL) in deionized water by BCA™ Protein Assay using a UV/V spectrophotometer (VersaMax, Molecular Devices, CA, USA) at a wavelength of 562 nm. Molecular Devices, LLC. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. After 72-h of incubation, CellTiter 96 Aqueous One Solution was added and cells were incubated for a further 2 h. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. This technical note is designed to provide information . SpectraMax® i3 Multi-Mode Detection Platform. This reliable model allows for. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. MOLECULAR DEVICES VersaMax is a customizable lab equipment capable of performing multiple types of research-grade tests and experiments at once, with enhanced safety protocols and adjustable column and sample-loading system for efficient sample preparation and increased throughput. S. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Cell. 0. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. 3 Methods 1. Molecular Devices SoftMax Pro 5. 4. . 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). Taking into account the price differences between each seller and each country. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Four wash/rinse bottles are included and configurable for both 96- and 384. . 0 out of 5 $ 2,999-20%. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. , USA) at 490 nm. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. 1 software, the leading microplate data acquisition and analysis software, you can download this PDF file from Molecular Devices. SoftMax® Pro, the industry-leading microplate reader software, is included at no. StakMax. Monday to Friday 8am - 5pm GMT. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The emodin thermoreversible PG was. developing diagnostic and research tools using. While the situation is vastly improving with numerous vaccine rollouts and over 6. , Sunnyvale, CA). (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. 9000-0756. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Then, the cultured cells were treated with different. The. Activity was calculated as picomoles of substrate hydrolyzed per min. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 4 (Molecular Devices cat. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. VCAM and ICAM were quantified by ELISA using a spectrophotometer (VERSAmax Molecular Devices) following the manufacturer’s protocol (DY643 and DY796, R&D Systems, Minneapolis, MN, USA). MiR-21. SpectraMax®, VersaMax, VMax. absorbance readers. Model: VERSAmax Tunable Microplate Reader. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. BMG Labtech - THERMOstar. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Download PDF versions of the SpectraMax Microplate Reader User Guides. This manual is subject to change without notice. 0, 0. VERSAmaxTM Tunable Microplate Reader Operator s Manual for Instruments with Serial Numbers Beginning. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. Molecular Devices also offers a mass spectrometry-based solution for food safety testing We would like to show you a description here but the site won’t allow us. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. We would like to show you a description here but the site won’t allow us. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. 3. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. Sale Sold out. used. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). After waiting 15 minutes for colour development at room temperature, the reaction was stopped with 1 M H 2 SO 4, and optical density values were read using an ELISA reader (Versamax, Molecular Devices, LLC, USA) at a wavelength of 492 nm, and the results were given as OD values. 2. Molecular Devices Spectramax Plate Reader. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. Molecular Devices E Max Precision Microplate Reader. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. 滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. Manufacturer: Molecular Devices; Includes PC Workstation w/ Softmax Pro Software Wavelength Range (nm): 340–850 Wavelength Accuracy: < ±0. P. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. OptiMax (Versamax non EXT) B0: Screenstation: all: Skanstacker: all: Skanwasher 300: all: Skanwasher 400: all: SpectraMax 190: N0:. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). Select Start>Control panel>Device Manager. With optimized reagents, option to operate the. . This. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. Additionally, the VersaMax Microplate Reader can be seamlessly integrated with the StakMax® Microlate Handling System through the SoftMax Pro Software. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. FAST AND ACCURATE METERTECH Microplate Reader $3,650. We would like to show you a description here but the site won’t allow us. This technical note is designed to provide information . 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). Unit price / per . Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. The absorbance was measured at 570 nm using a microplate reader (VersaMax, Molecular Devices). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. VERSAMAX. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax. StakMax. The absorbance of reaction mixtures was read at 475 nm using a microplate reader (VERSAMax, Molecular Devices) to determine the quantity of dopachrome produced. HepG2 cells were seeded into 6-well plates. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. Concentration was determined by BCA Protein Assay Kit (ref. StakMax Microplate Handling System. TECHNICAL NOTE. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. All samples were measured in triplicate. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. . Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. Culture medium was removed, and lysis buffer (10 ml) was pipetted into each well. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Bacterial growth was determined turbimetrically using the microplate ELISA reader VERSAmax (Molecular Devices). Wavelength Range. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Multichannel optics. Cell viability was quantified by microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA) at 570 nm. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. SpectraMax® Microplate Reader User Manual Download Page. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. 68 $. For more sample throughput, the system can be easily integrated into a full roboticThe VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. used. Unit price / per . Access all the resources, tools and information you need to stay productive. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax,Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. 5°C @ 37°C Well-to. Used. 00 Regular price. All. 8). Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. Find regional support. Louis, USA) was used to. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. reader (VersaMax, Molecular devices, San Jose, CA, USA). 5 mM. Tunable, filterless flexibility The. Proliferation of the released daughter cells was monitored at a wavelength of 578 nm online by a microplate reader (VersaMax; Molecular Devices, Sunnyvale, Calif. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. We would like to show you a description here but the site won’t allow us. Wavelength Selection. In general terms, plasma was added to a 96-well plate containing the targeted antisera, after rigorous washing steps and conjugation with a secondary detection antisera, and the resultant colorimetric reaction was read by scanning spectrophotometry (VersaMax, Molecular Devices, Sunnyvale, CA). 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). Versamax Microplate Reader dibangun di sekitar monokromator kisi. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). 2. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. ). 5 mM was used to make the. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. microtiter plate reader (VERSAmax, Molecular Devices). Whether bacteria were partially or completely inactivated by bone cement. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. Ann Arbor, MI, USA). reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. An ELISA plate reader (VERSAmax; Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at 480 nm. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. $1,195. For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. $495. With optimized reagents, option to operate the. Offer Ends In: 5 Days 05: 02: 03. The water extract afforded the greatest yield, and total phenolic and tannin. Absorbance plate reader (e. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 파장 범위(nm) : 340–850. Molecular Devices Corporation. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of delivery. 0 out of 5 $ 2,999. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). 5) containing 0. 파장 정확도 : < ±0. 키네틱 읽기 시간 : 최소 9초 간격. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan Nihon Molecular Devices Osaka Tel. Molecular Devices VersaMax Tunable Microplate Reader. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of. 2. To make your request, nothing more simple: you just have to connect on the site and to make your request on line. , VersaMax; Molecular Devices) Incubator for cell culture. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. 2. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Formerly known as MicroMax, Molecular Devices’ unique SpectraDrop™ Micro-Volume Microplate, offers the highest throughput solution for low volume measurement available onthe market today. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. Model : VersaMax . Cyanobacterial strains that did not lyse until the 12th day were defined as insusceptible. 2 and 648. All the experiments were conducted at. 1700 +1 800. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. HT22 cells are derived from hippocampal neuronal HT4 cells. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. 4 Microplate Reader Software w WARRANTY. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. These models can no longer be repaired. The. EC migration was studied by wound healing assay. The amount of MTT reduction was measured immediately by detecting absorbance in a microplate reader (Versamax, Molecular Devices) at a wavelength of 570 nm and a reference wavelength. Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Approximately. Table of Contents. p-nitrophenol (Sigma) with diluted concentrations ranging from 0 to 0. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). SpectraTest ABS1 Absorbance Validation Plate. 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). The final mixtures were allowed to react at 25 °C in the dark for 5 min. This technical note is designed to provide information . (VersaMax, Molecular Devices, USA). To date, over 219 million cases and 4. Western Blot. The BMDM cells were plated into a 6-well culture plate at a density of 2. MDC instruments including: SpectraMax®, VersaMax. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. SpectraTest ABS1 Absorbance Validation Plate. 0 N sulfuric acid. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Used. Intracellular triglyceride assay. 03 billion doses of. Measurement of brain-derived neurotrophic factor (BDNF) release. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. . 0. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. Finally, the absorbance was determined at 490 nm on a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA). This document is provided to customers who have purchased Molecular Devices, Inc. 00. Total. For validation of the Molecular Devices VersaMax™ Absorbance Microplate Reader for use with the Pyrochrome Assay, data acquisition and analysis were performed with SoftMax ® Pro GxP Software. HT22 cells are derived from hippocampal neuronal HT4 cells. Molecular Devices SoftMax Pro 5. The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. 47 shipping. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. 6/10 #0120-1330d. Turn the reader on and secure the USB cable. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The value for non-specific absorption at 600 nm was subtracted. SpectraMax Plus384, 190, 340PC384, and VersaMax User Guide Molecular Devices User Guide SpectraMax. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high-qualityspectrophotometer. 2 and 648. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software, SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. reader (VersaMax, Molecular devices, San Jose, CA, USA). The. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both. Sterile AB medium and a mixture of Me 2 SO, methanol, and water (ratio v/v 1:1:8) served as negative controls. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. A. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. View the supported Window operating systems for the different versions of the SoftMax Pro software. 2. 2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. Cell proliferation assay MDA-MB-231 cells were seeded at a density. It also integrates with the. 4. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Access this by going to. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. If you are looking for a comprehensive user guide for SoftMax Pro 7. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. , Sunnyvale, CA). harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. VersaMax ELISA Microplate Reader. It covers everything from installation, configuration, data acquisition, data analysis, curve fitting, to reporting and troubleshooting. S. Used. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. ® ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The TBARS content was calculated according to the extinction coefficient as 155 mM −1 cm −1. If the computer is connected to the Internet: Select Search Automatically for. 0 (Molecular Devices, Sunnyvale, CA). One microgram of RNA was. 0 OD. Windows 10 – 2004/20H2/21H1/21H2. 0A Sunnyvale, CA Made in USA VersaMax price. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. Spectrophotometers. Also see: SoftMax® Pro Microplate Software User Manual Download Page. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. A. DNA content in the supernatant (100 μl) was measured using a Quant-iT PicoGreen assay kit (Invitrogen) as per the manufacturer's instructions. Microplate Reader Shelf. The SpectraTest® ABS1 Absorbance. Ensure data accuracy of your reader. 1. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. The percentages of inhibition of cell viability were calculated as the ratio between the absorbance of the compound-treated cells and cells with only the vehicle. [Google. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . A. s. +44-118-944-8000. the plate reader (model VERSAmax, Molecular Devices, USA) at 515 nm (A515). The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. The sections were stained with hematoxylin and eosin (H&E) and. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. $1,749. Primer design. ImmunohistochemistryAfter 1 h incubation at 37 °C, the color intensity was measured at 562 nm by a microplate reader (model VERSAmax; Molecular Devices, San Jose, CA, USA). The culture supernatant of cells (25 μl) was mixed with 225 μl of DMMB dye solution and the absorbance of solution mixture was measured at 525 nm with a spectorophotometer (VERSAmax; Molecular Devices Japan KK, Tokyo, Japan). This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. Get pricing and purchase lab equipment and. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). StakMax Microplate Handling Stacker.